Anti-Mannan-binding lectin (MBL, chicken)
Mouse monoclonal antibody
Cat.No. HYB 182-01
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Mannan-binding lectin (MBL) belongs to the C-type family of collectins. It functions as an opsonin which activates complement on binding to microbial polysaccharides. Studies on chicken MBL are described in refs 1-4
MBL purified from chicken plasma and adsorbed onto aluminum hydroxide
HYB 182-01 is specific for chicken MBL
Not determined
In ELISA HYB 182-01 reacts strongly with chicken MBL. HYB 182-01 can be used as coating antibody (5 µg/mL) with biotinylated HYB 182-01 as detection antibody (1 µg/mL) in sandwich ELISA (1-3)
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/4000 | 3 |
| Immunoblotting | Yes | 1/1000 | 4 |
| Immunohistochemistry | Yes | 1/50 | 4 |
The dilution guideline for ELISA is based on use as detection antibody for antigen bound via mannan coated at 3 µg/mL mL together with human albumin 8.5 µg/mL onto the microtiter well. Users should determine the optimal dilutions for their own purposes.
1.Laursen SB, Hedemand JE, Nielsen OL, Thiel S, Koch C, Jensenius JC (1998) Serum levels, ontogeny and heritability of chicken mannan-binding lectin (MBL). Immunology 94:587-593.
2. Nielsen OL, Jensenius JC, Jorgensen PH, Laursen SB (1999) Serum levels of chicken mannan-binding lectin (MBL) during virus infections; indication that chicken MBL is an acute phase reactant. Vet Immunol Immunopathol 70:309-316.
3. Juul-Madsen HR, Munch M, Handberg KJ, Sorensen P, Johnson AA, Norup LR, Jorgensen PH (2003) Serum levels of mannan-binding lectin in chickens prior to and during experimental infection with avian infectious bronchitis virus. Poult Sci 82:235-241.
4. Nielsen OL, Jorgensen PH, Hedemand J, Jensenius JC, Koch C, Laursen SB (1998) Immunohistochemical investigation of the tissue distribution of mannan-binding lectin in non-infected and virus-infected chickens. Immunology 94:122-128.
Downloads
HYB 182-01.pdf (19.91 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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